Restriction digests of the clone give the following sizes (kb): BamHI--6.4; SacI/XbaI--3.2, 2.8, 0.42; XhoI--6.4.
High copy number shuttle expression vector.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References
Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504
