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Fgf8 cDNA1 [mFgf8]

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價格:詢價

編號:B178105

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

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產品名稱

Fgf8 cDNA1 [mFgf8]

商品貨號

B178105

Designations

Fgf8 cDNA1 [mFgf8]

GenBank Number

Z46883

培養基

Species

Mus musculus, mouse

Depositors

C Murphy, GR Martin

Applications

To prepare an antisense probe, cut the plasmid with PstI and use T7 RNA polymerase.

Vector

Construct size (kb): 3.799999952316284

Insert

DNA: cDNA

Insert lengths(kb): 0.800000011920929

Gene product: fibroblast growth factor-8(androgen-induced growth factor) [AIGF]

Alleles: b, b, s, s

Insert Size (kb)

0.800

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): SmaI/XhoI--3.0, 0.8; SmaI--3.8; XhoI--3.8; NcoI--3.65, 0.15; PstI--3.8.

The insert contains the following restriction sites (approximate kb from the 5' end): BalI--0.14; NcoI--0.16; StyI--0.16.

This insert is an alternate splice variant of Fgf8 that corresponds to bp 1-35 and 906-1038 of Z46883.

To prepare an antisense probe, cut the plasmid with PstI and use T7 RNA polymerase.

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Crossley PH, Martin GR. The mouse Fgf8 gene encodes a family of polypeptides and is expressed in regions that direct outgrowth and patterning in the developing embryo. Development 121: 439-451, 1995. PubMed: 7768185

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