U67060
Construct size (kb): 10.0
Intact vector size: 6.0
Type of vector: phagemid
Vector end: ClaI
Vector end: NdeI
Cloning sites: ClaI NdeI
Cloning capacity: pcDNA3, TK, HSV
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
promoter for expression: TK, ->
restriction site: NotI
promoter for in vitro transcription: T7, ->
stop codons: TAG, ->
restriction site: SpeI NheI
initiation codon: ATG, ->
epitope tag: HSV, ->
restriction site: ClaI
restriction site: NdeI
epitope tag: T7, ->
stop codons: TGA, ->
promoter for in vitro transcription: SP6, <-
terminator: bGH polyadenylation, ->
replicon: f1, ->
replicon: SV40, ->
promoter: SV40, ->
marker(s): G418R, ->
terminator: SV40 polyadenylation, ->
replicon: pMB1
marker(s): ampR, <-
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195
Joseph L Goldstein, personal communication
