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pGEM-hJE34
pGEM-hJE34
規格:
價格:詢價
編號:B178274
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pGEM-hJE34
商品貨號
B178274
Designations
pGEM-hJE34
GenBank Number

M26035

培養基
Species
Homo sapiens, human
Depositors
BJ Rollins
Vector
Construct size (kb): 3.740000009536743
Insert
DNA: cDNA
Insert lengths(kb): 0.7400000095367432
Tissue: embryonic lung fibroblast WI-38 (serum-induced)
Gene product: cytokine A2, small inducible( monocyte chemotactic protein, monocyte chemotactic protein 1, homologous to mouse Sig-je, small inducible cytokine A2, small inducible cytokine subfamily A (Cys-Cys), member 2, small inducible gene JE) [JE*]
Insert Size (kb)
0.740
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The insert includes the following sites (with nucleotide positions according to the published sequence): BglII--193, 286; NcoI--325; PstI--397; SpeI--539.
Restriction digests of the clone give the following sizes (kb): XbaI--3.7; BglI--3.7; PstI--3.0, 0.35 (doublet); SacI--3.7; EcoRI/BamHI--3.0, 0.75; XhoI--3.0, 0.75; KpnI--3.0, 0.75.
This insert was subcloned from pXM-hJE34, so the KpnI insert includes XhoI, SacI, and PstI sites at the 5' end and XhoI and PstI sites at the 3' end from pXM. The insert includes 24 nucleotides of poly(A) tail.
The insert is oriented such that transcription from the SP6 promoter gives the sense strand.
References

Rollins BJ, et al. The human homolog of the JE gene encodes a monocyte secretory protein. Mol. Cell. Biol. 9: 4687-4695, 1989. PubMed: 2513477

Rosl F, et al. Differential regulation of the JE gene encoding the monocyte chemoattractant protein (MCP-1) in cervical carcinoma cells and derived hybrids. J. Virol. 68: 2142-2150, 1994. PubMed: 8138998

Barrett J Rollins, personal communication

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