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Primary Subcutaneous Pre-adipocytes; Normal, Human

Primary Subcutaneous Pre-adipocytes; Normal, Human

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編號:B178456

品牌:Mingzhoubio

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產品名稱

Primary Subcutaneous Pre-adipocytes; Normal, Human

商品貨號

B178456

Organism

Homo sapiens, human

Tissue

Mature adipose tissue

培養基

Morphology

Spindle-shaped, fibroblast-like

Growth Properties

Adherent

Biosafety Level

1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

Adult

Gender

Lot-specific

Ethnicity

Lot-specific

Applications

Adult stem cell differentiation, regenerative medicine, cell therapy, tissue engineering, creation of iPS cell lines

Product Format

frozen 1 mL

Storage Conditions

-130°C or below

Comments

ATCC^® Human Pre-Adipocytes are cryopreserved at the second passage to ensure the highest viability and plating efficiency. ATCC^® Primary Cell Solutions™ cells, media, supplements and reagents are quality tested together to guarantee optimum performance and reliability.

Complete Growth Medium

Obtain one Fibroblast Growth Kit-Low Serum from the freezer; make sure that the caps of all components are tight.
Thaw the components of the growth kit just prior to adding them to the basal medium.
Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer.

Table 1. Fibroblast Growth Kit-Low Serum Components (ATCC^® PCS-201-041)

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Component

Subculturing

Volume

Volume

Final Concentration

Cells per Vial

rh FGF β

Sterility Tests

0.5 mL

Viral Testing

5 ng/mL

Viability

L-glutamine

C of A

18.75 mL

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