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pJA50

規格:

價格:詢價

編號:B178508

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pJA50

商品貨號

B178508

Designations

pJA50

Depositors

SJ Elledge

培養基

Biosafety Level

Vector Information

Size (kb): 6.5409998893737790

Vector: pJA50 (phagemid)

Promoters: Promoter lac

Construction: pUC

Marker(s):ampR,kanR,HIS3

Construct size (kb): 6.540999889373779

Features: insert detection: lacZ'
marker(s): ampR, kanR, HIS3
promoter: lac
replicon: pMB1, f1

Applications

shuttle vector

vector for other uses

vector permitting visual detection of recombinants

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--6.54; PstI--5.1, 0.7, 0.7; HindIII--4.1, 1.8, 0.65.

Shuttle vector to facilitate construction of gene disruptions in E. coli. The gene of interest is cleaved at an appropriate site, made flush, and ligated to the SmaI fragment from this plasmid that contains kanR and the yeast selectable marker.

The appropriate product is selected for by kanR in E. coli.

The order of the major features in this plasmid is: kanR - HIS3 - EcoRI/MCS/HindIII - f1 ori - ampR - pMB1 ori.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Stephen J Elledge, personal communication

Related Products

component of:ATCC 77255

Shipped

freeze-dried

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