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pLTRdhfr26

規(guī)格:

價格:詢價

編號:B178614

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pLTRdhfr26

商品貨號

B178614

Designations

pLTRdhfr26

Depositors

RA Weinberg

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 8.6000003814697270

Vector: pLTRdhfr26 (plasmid)

Promoters: Promoter Ha-SV LTR

Construction: pBR322, pDHFR26, HaSV clone H-1

Marker(s):ampR,mtxR (Dhfr)

Construct size (kb): 8.600000381469727

Features: marker(s): ampR, mtxR
promoter: Ha-SV LTR
replicon: pMB1

Applications

contains sequence dihydrofolate reductase

expression vector

integrating vector

shuttle vector

vector permitting positive selection for integration

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--8.6, 1.36; EcoRI--9.4; HindIII--9.4.

Transfected methotrexate-resistant NIH 3T3 cells carried double minute chromosomes that appeared to contain DNA acquired during transfection.

Can be used in cotransfection experiments to generate a selectable methotrexate-resistant phenotype.

A 3.3 kb EcoRI/BamHI fragment of HaSV clone H-1 (including the LTR) was cloned into EcoRI/BamHI-digested pBR322. A PstI fragment with mouse Dhfr (with BamHI linkers) was then cloned into the BamHI site of the construct.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Murray MJ, et al. Construction and use of a dominant, selectable marker: a Harvey sarcoma virus-dihydrofolate reductase chimera. Mol. Cell. Biol. 3: 32-43, 1983. PubMed: 6298606

Shipped

freeze-dried

Shipping Information

Distributed: freeze-dried

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