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c9196
c9196
規格:
價格:詢價
編號:B179205
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
c9196
商品貨號
B179205
Designations
c9196
GenBank Number

U11583

培養基
Species
Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors
L Riles, MV Olson
Vector
Construct size (kb): 43.20000076293945
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: CBP2
Gene name: intron processing factor
Contains complete coding sequence?: Y
Chromosome: VIII; Localization: VIII L
Genome: Saccharomyces cerevisiae
Gene symbol: SSB1
Gene name: single-strand nucleic acid binding protein
Contains complete coding sequence?: Y
Chromosome: VIII
Genome: Saccharomyces cerevisiae
Gene symbol: RPL4A
Other gene symbols: MAK7
Gene name: ribosomal protein L7A-1
Contains complete coding sequence?: Y
Chromosome: VIII; Localization: VIII L, 44 cM from CEN
Genome: Saccharomyces cerevisiae
Gene symbol: GUT1
Gene name: glycerol kinase
Contains complete coding sequence?: Y
Chromosome: VIII; Localization: VIII L
Genome: Saccharomyces cerevisiae
Gene symbol: RIM1
Gene name: positive regulator of meiosis
Contains complete coding sequence?: Y
Chromosome: VIII
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 36.792
Cross references: DNA Seq. Acc.: U11583
Nucleotides 1-44587 of the insert correspond to
nucleotides 10483-55069 of U11583.
Insert lengths(kb): 36.79199981689453
Gene product: glycerol kinase [CBP2]
Insert Size (kb)
36.792
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--14.0, 5.0, 4.0 (doublet), 2.15, 1.8 (doublet), 1.5, 1.25 (triplet), 1.15 (doublet), 1.1 (doublet), 0.95 (doublet), 0.9 (doublet), 0.7 (triplet), 0.6 (doublet), 0.5.
Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 4.952, 4.182, 4.166, 2.253, 1.848, 1.848, 1.512, 1.247, 1.247, 1.241, 1.163, 1.163, 1.100, 1.100, 0.948, 0.912, 0.912, 0.869, 0.729, 0.713, 0.700, 0.616, 0.574, 0.477.
In an EcoRI/HindIII digest of the cosmid, the vector fragment is 13,365 kb.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Corresponds to nt 11143-47935 of the sequence of chromosome VIII.
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229

Linda Riles, personal communication

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