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pNF3
pNF3
規格:
價格:
編號:B179538
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pNF3
商品貨號
B179538
Designations
pNF3
Depositors
PA Scolnik
培養基
Applications
expression vector
shuttle vector
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--8.2, 1.0; HindIII--8.2, 1.0.
A wide range of transcription levels can be obtained by varying the nitrogen source in the medium. Transcription can be immediately stopped by the introduction of oxygen and ammonia.
The spcR marker is expressed in R. capsulatus.
A Rhodobacter capsulatus expression vector with transcription of the insert under the control of the nifH promoter.
References

Pollock D, et al. Transcription of the rhodobacter capsulatus nifHDK operon is modulated by the nitrogen source. Construction of plasmid expression vectors based on the nifHDK promoter. Gene 65: 269-275, 1988. PubMed: 3410321

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