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當(dāng)前位置: 首頁 > ATCC代理 > p220 [pPE220] B180098
p220 [pPE220]
p220 [pPE220]
規(guī)格:
價格:詢價
編號:B180098
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
p220 [pPE220]
商品貨號
B180098
Designations
p220 [pPE220]
Species
Mus musculus, mouse
培養(yǎng)基
Depositors
RW Elliott
Vector
Construct size (kb): 3.299999952316284
Insert
DNA: cDNA
Insert lengths(kb): 0.6000000238418579
Tissue: parietal endoderm
Gene product: secreted protein, acidic, cysteine-rich (osteonectin) [Sparc]
Alleles: b, b, d, b, d, d
Insert Size (kb)
0.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The insert contains the following restriction sites (in approximate bp from the 5' end): EcoRI--0; KpnI--250; NcoI--300; PstI--444.
Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--2.8, 0.45, 0.17; EcoRI/HindIII--2.8, 0.58; KpnI--3.3; EcoRI--3.3; HindIII--3.3; EcoRI/SalI--3.3; SalI--uncut.
The BglII, PstI, and BamHI polymorphisms all have the same allele segregation pattern, suggesting a single mutational event.
This detects the following genomic restriction fragments (kb): HindIII--16; TaqI--3.7, 2.8; EcoRI--7.5, 1.0 (3T3) or 7.5, 6.2 (RTM9); BamHI--4.2, 2.4 (3T3) or 4.7, 3.0 (RTM9).
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Mason IJ, et al. Developmental and transformation-sensitive expression of the Sparc gene on mouse chromosome 11. EMBO J. 5: 1831-1837, 1986. PubMed: 3758028

Mason IJ, et al. Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell 'culture shock' glycoprotein of Mr 43000. EMBO J. 5: 1465-1472, 1986. PubMed: 3755680

Holland PW, et al. In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization. J. Cell Biol. 105: 473-482, 1987. PubMed: 2440898

McVey JH, et al. Characterization of the mouse SPARC/osteonectin gene. J. Biol. Chem. 263: 11111-11116, 1988. PubMed: 3165375

Rosemary W Elliott, personal communication

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