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pEAD

規格:

價格:詢價

編號:B180711

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

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產品名稱

pEAD

商品貨號

B180711

Designations

pEAD

GenBank Number

V01555

培養基

Species

Human gammaherpesvirus 4 (HHV-4), Epstein-Barr virus

Depositors

Johns Hopkins University, G Milman, Johns Hopkins University

Applications

produces protein early antigen, diffuse (open reading frame BMRF1)

Vector

Construct size (kb): 8.34000015258789

Insert

DNA: genomic

Insert lengths(kb): 4.340000152587891

Tissue: B95-8 cell line

Gene product: early antigen, diffuse (open reading frame BMRF1) [EBEAD]

Target Gene: early antigen, diffuse (open reading frame BMRF1)

Insert Size (kb)

4.340

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): BglII--6.6, 1.6; EcoRI--8.2; XbaI--8.2.

The insert contains the following restriction sites (approximate kb from the 5' end): BglII--1.34; EcoRI--3.03; HindIII--2.19; KpnI--1.42; NotI--0.91; PstI--0.10, 3.05, 4.23; SacI--3.78; SmaI--0.95, 1.82, 3.84; XbaI--1.96; XhoI--1.79, 3.73.

pHE6 is a high-expression plasmid derived from pUC8. Inserts are expressed as run-on products initiated at lambda N. Synthesis is regulated by the cI857 repressor encoded by the plasmid.

Corresponds to nt 79895-84233 of the GenBank record.

Expresses a 47 kDa fusion polypeptide composed of 33 amino acids from the lambda N protein and the BMRF1 open reading frame. This is recognized by monoclonal antibody R3.

Classification

Herpesviridae, Lymphocryptovirus

References

Milman G. Expression of antigenic epstein-barr virus polypeptides in bacteria and their use in diagnostics. US Patent 5,256,768 dated Oct 26 1993

Cho MS, et al. A second Epstein-Barr virus early antigen gene in BamHI fragment M encodes a 48- to 50-kilodalton nuclear protein. J. Virol. 56: 860-866, 1985. PubMed: 2999442

Baer R, et al. DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 310: 207-211, 1984. PubMed: 6087149

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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