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pASK84

規格:

價格:詢價

編號:B180730

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pASK84

商品貨號

B180730

Designations

pASK84

Depositors

A Skerra

培養基

Biosafety Level

Vector Information

Size (kb): 4.5869998931884770

Vector: pASK84 (plasmid)

Promoters: Promoter for expression lacUV5

Construction: pASK30, pASK37-IgG1

Marker(s):ampR

Construct size (kb): 4.586999893188477

Features: leader peptide: phoA
marker(s): ampR
promoter for expression: lacUV5
replicon: f1
replicon: pMB1
repressor gene: lacI
restriction site: HindIII
restriction site: NcoI
restriction site: XbaI
signal peptide coding region: ompA
terminator: lpp
coding sequence: Ig gamma 1 constant region
coding sequence: Ig kappa constant region
epitope tag: hexahistidine sequence (6-His)

Applications

expression vector

vector permitting construction of fusion proteins

vector with sequences for protein export

Comments

Restriction digests of the clone give the following sizes (kb): NcoI/XbaI--4.0, 0.5; HindIII/NcoI--4.0, 0.5; EcoRI--4.6.

Contains part of the hinge region of immunoglobulin gamma 1.

The heavy chain and light chain variable regions may be cloned into the PstI-EcoRI-BstEII and SstI-PmlI-XhoI multiple cloning sites respectively.

The other unique restriction sites (XbaI NcoI HindIII) may be used for subcloning or exchange of immunoglobulin constant regions.

Immunoglobulin expression vector allowing cloning of variable region domains and efficient purification of Fab fragments via immobilized metal affinity chromatography.

Contains the immunoglobulin heavy chain region, part of the hinge region and the immunoglobulin kappa constant region.

The products are secreted into the periplasm where protein folding and disulfide bond formation occurs.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Skerra A. A general vector, pASK84, for cloning, bacterial production, and single-step purification of antibody Fab fragments. Gene 141: 79-84, 1994. PubMed: 8163179

Arne Skerra, personal communication

Shipped

freeze-dried

Shipping Information

?Freeze dried Escherichia coli containing the plasmid.

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