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F814
F814
規格:
價格:詢價
編號:B180918
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
F814
商品貨號
B180918
Designations
F814
Species
Homo sapiens, human
培養基
Applications
This probe can be used routinely to detect both the F8C and DXS52 (VNTR) RFLPs on 0.9 - 1.0 agarose gels.
Vector
Construct size (kb): 4.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 1.5
Gene product: coagulation factor VIIIc, procoagulant component (hemophilia A) [F8C]
Alleles: F3, E1, E1, E2, F1, F2, B1, F1, F2, A1, A2, B2
Insert Size (kb)
1.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.2; EcoRI/HindIII--2.7, 1.5; HindIII--2.7, 1.5; PstI--3.5, 0.68; XbaI--4.2.
Linkage has been demonstrated between X-linked recessive hydrocephalus (HSAS) and DXS52 (theta = 0.03, LOD score = 6.52). Linkage has also been demonstrated between HSAS and F8 (theta = 0.00, LOD score = 4.32).
This is a hybrid probe containing a fragment of the factor VIII gene that detects the BclI RFLP and a fragment close to the St14 minisatellite that detects the VNTR in several restriction digests including TaqI, BclI, and MspI.
This probe can be used routinely to detect both the F8C and DXS52 (VNTR) RFLPs on 0.9 - 1.0 agarose gels. It also gives a clear pattern for DXS52 on TaqI blots.
This probe does not detect the 2-allele RFLPs of St14-1 (ATCC 61372) (TaqI, BclI).
References

Mandel JL, et al. Genetic mapping of the human X chromosome: linkage analysis of the q26-q28 region that includes the fragile X locus and isolation of expressed sequences. Cold Spring Harbor Symp. Quant. Biol. 51: 195-203, 1986. PubMed: 3472716

Heilig R, et al. Improved DNA markers for efficient analysis of fragile X families. Am. J. Med. Genet. 30: 543-550, 1988. PubMed: 2902795

Vincent A, et al. The polymorphic marker DXS304 is within 5 centimorgans of the fragile X locus. Genomics 5: 797-801, 1989. PubMed: 2574147

Willems PJ, et al. Assignment of X-linked hydrocephalus to Xq28 by linkage analysis. Genomics 8: 367-370, 1990. PubMed: 1979056

Jean Louis Mandel, personal communication

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