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pCMV HSV S2P plasmid in <em>Escherichia coli</em>

規格:

價格:詢價

編號:B181046

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pCMV HSV S2P plasmid in Escherichia coli

商品貨號

B181046

Designations

pCMV HSV S2P plasmid in Escherichia coli

GenBank Number

AF019612

培養基

Species

Homo sapiens, human

Depositors

JL Goldstein

Applications

encodes an epitope tag for protein isolation or monitoring S2P

Vector

Vector: pcDNA3
Vector type: phagemid
Vector size (kb): 5.4
Markers: ampR
Features: CMV promoter, T7 and SP6 promoters, SV40 replicon, G418 resistance, pMB1 replicon

Insert

Insert size (kb): 1.7
Gene: S2P
DNA: cDNA
Source : human
Genbank accession: AF019612
Insert end 5’: NheI
Insert end 3’: XbaI

Insert Size (kb)

1.7

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Freeze dried E. coli containing the plasmid

Comments

Restriction digests of the clone give the following sizes (kb): NheI/XbaI--5.4, 1.7; PstI--4.0, 3.1; ClaI--7.1; EcoRI--7.1.

The insert contains the following restriction sites (approximate kb from the 5' end): StyI--0.36; DraII--0.90; BglI--1.40; SacI--1.46.

The insert contains human S2P with HSV epitope tag at the amino terminus.

References

Rawson RB, et al. Complementation cloning of S2P, a gene encoding a putative metalloprotease required for intramembrane cleavage of SREBPs. Mol. Cell 1: 47-57, 1997. PubMed: 9659902

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