Vector Information
Size (kb): 3.3810000419616700
DESCRIPTION OF VECTOR:
Intact vector size: 3.381
Type of vector: phagemid
Cloning sites: EcoRI SacI Asp718 KpnI SmaI BamHI
Polylinker sites: EcoRI SacI Asp718 KpnI SmaI BamHI
Construction: pUK21, pET3b, pEZZ18
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7, ->, 82-98
ribosome-binding site: Shine-Dalgarno, ->, 148-153
restriction site: NdeI, 159-164
leader peptide: synthetic ZZ sequence of protein A, ->, 162-641
MCS: EcoRI...BamHI, ->, 642-670
transcription terminator: T7, ->, 673-795
other: M13 intergenic region, ->
marker(s): kanR, ->
replicon: M13
Vector: pExSec1 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pUK21, pET3b, pEZZ18
Marker(s):kanR
Construct size (kb): 3.38100004196167
Features: leader peptide: synthetic ZZ sequence of protein A
marker(s): kanR
other: M13 intergenic region
promoter for in vitro transcription: T7
replicon: M13
MCS: EcoRI...BamHI
restriction site: NdeI
ribosome-binding site: Shine-Dalgarno
transcription terminator: T7
