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pTZA1
pTZA1
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B181696
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pTZA1
商品貨號(hào)
B181696
Designations
pTZA1
GenBank Number

X13294

培養(yǎng)基
Species
Homo sapiens, human
Applications
contains sequence avian myeloblastosis viral (v-myb) oncogene homolog-like 1
Vector
Construct size (kb): 7.800000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTZ19
Intact vector size: 2.900
Type of vector: phagemid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC19, Escherichia coli lacZalpha, pBR322, f1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MYBL1
Gene name: avian myeloblastosis viral (v-myb) oncogene homolog-like 1
Contains complete coding sequence?: Y
Chromosome: 8; Localization: 8 q22
Tissue: B-lymphocytes
Type of DNA: cDNA
Insert 5' end: BamHI
Insert 3' end: EcoRI
Insert size (kb): 4.9
Cross references: DNA Seq. Acc.: X13294
Nucleotides 1-2317 of the insert correspond to
nucleotides 25-2341 of X13294.
DNA Seq. Acc.: X66087
Nucleotides 1270-4857 of the insert correspond to
nucleotides 1-3587 of X66087.
Insert lengths(kb): 4.900000095367432
Tissue: B lymphocyte
Gene product: avian myeloblastosis viral (v-myb) oncogene homolog-like 1 [MYBL1]
Target Gene: avian myeloblastosis viral (v-myb) oncogene homolog-like 1
Insert Size (kb)
4.900
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--3.6, 3.0, 0.62, 0.35, 0.21, 0.19; EcoRI--3.6(doublet), 0.35, 0.23, 0.19; HindIII--4.8, 3.1; PstI--5.3, 2.4, 0.21; PvuII--4.3, 2.6, 1.1.
Insert contains the following restriction sites (approximate kb from the 5' end): BglI--1.13; BglII--1.74; EcoRI--0.64, 0.96, 1.11, 1.29; EcoRV--2.31; HindIII--3.11; KpnI--1.75, 3.45; NdeI--0.75; NcoI--1.31; PstI--2.40, 2.69; PvuII--0.93; SacI--2.35.
There are internal EcoRI sites. The coding portion of the insert can be excised with HindIII.
References

Golay J, et al. The human A-myb protein is a strong activator of transcription. Oncogene 9: 2469-2479, 1994. PubMed: 8058310

Josee Golay, personal communication

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