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pAKA16

規格:

價格:詢價

編號:B182234

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pAKA16

商品貨號

B182234

Designations

pAKA16

Depositors

JG Coote, AK Azad

培養基

Biosafety Level

Vector Information

Size (kb): 5.3000001907348630

Vector: pAKA16 (plasmid)

Construction: pPH843, pIC20H

Marker(s):ampR

Construct size (kb): 5.300000190734863

Features: insert detection: lacZ'
marker(s): ampR
other: mob
replicon: ori(P)
MCS: HindIII/SphI/PstI/.../NruI/HindIII

Applications

shuttle vector

vector permitting visual detection of recombinants

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--5.3; BamHI--5.3; PstI--3.5, 1.75.

The unique Bsp1286I is located within the ori(P).

Shuttle vector that can be mobilized by conjugation between E. coli and Pasteurella haemolytica.

Constructed by ligating a 979 bp ApaLI fragment from pIC20H (which contains lacZalpha, MCS, and lacI'PO) to ApaLI-digested pPH843.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Azad AK, et al. Construction of conjugative shuttle and suicide vectors for Pasteurella haemolytica and P. multocida. Gene 145: 81-85, 1994. PubMed: 8045428

Azad AK, et al. Distinct plasmid profiles of Pasteurella haemolytica serotypes and the characterizaton and amplification in Escherichia coli of ampicillin-resistance plasmids encoding ROB-1 beta-lactamase. J. Gen. Microbiol. 138: 1185-1196, 1992. PubMed: 1527493

Shipped

freeze-dried

Shipping Information

?Freeze dried E. coli containing the plasmid

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