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pDRD2E5.6

規格:

價格:詢價

編號:B183224

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pDRD2E5.6

商品貨號

B183224

Designations

pDRD2E5.6

Species

Homo sapiens, human

培養基

Depositors

JR Kidd

Vector

Construct size (kb): 8.5600004196167

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7, T3
replicon: pMB1, f1
Cross references:

Insert

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DRD2
Genomic copy number: unique
Gene name: dopamine receptor D2
Contains complete coding sequence?: U
Chromosome: 11; Localization: 11 q22.2-q22.3
Type of DNA: genomic
Insert 5' end: EcoRI
Insert 3' end: EcoRI
Insert size (kb): 5.6
Cross references:

Insert lengths(kb): 5.599999904632568

Gene product: dopamine receptor D2 [DRD2]

Alleles: A1, B1, B2, A2

Insert Size (kb)

5.600

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 3.0; BamHI--8.0, 0.6; HindIII--8.6; PstI--4.0, 1.5, 1.35, 0.8, 0.5, 0.3; HindIII/PstI--3.4, 1.5, 1.3, 0.8, 0.8, 0.5, 0.3.

The insert contains a 4.5Kb TaqI fragment.

The downstream portion of pDRD2E5.6 overlaps the Suarez clone phD2-254.

References

Hauge XY, et al. Detection and characterization of additional DNA polymorphisms in the dopamine D2 receptor gene. Genomics 10: 527-530, 1991. PubMed: 1679742

Suarez BK, et al. Linkage Disequilibria at the D2 dopamine receptor (DRD2) in alcoholics and controls. Genomics 19: 12-20, 1994. PubMed: 7910578

Judith R Kidd, personal communication

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