最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pGEM4Z/SEAP B183258
pGEM4Z/SEAP
pGEM4Z/SEAP
規格:
價格:詢價
編號:B183258
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pGEM4Z/SEAP
商品貨號
B183258
Designations
pGEM4Z/SEAP
Species
Homo sapiens, human
培養基
Depositors
S Udenfriend
Applications
If the plasmid is linearized with HindIII, and transcribed from the SP6 promoter in the presence of m7-GpppG, mRNA for the secreted form of placental alkaline phosphatase is produced.
mRNA prepared from this construct can be used as an internal standard for expression of mRNA injected into Xenopus oocytes.
Vector
Construct size (kb): 5.0
Insert
DNA: cDNA
Insert lengths(kb): 2.0
Gene product: alkaline phosphatase, placental (Regan isozyme) [ALPP]
Alleles: A1, A2, B1, B2
Insert Size (kb)
2.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; HindIII--5.2; BamHI--3.2, 1.8; PstI--3.2, 1.8; SalI--5.2; EcoRI/KpnI--2.9, 2.1.
If the plasmid is linearized with HindIII, and transcribed from the SP6 promoter in the presence of m7-GpppG, mRNA for the secreted form of placental alkaline phosphatase is produced.
mRNA prepared from this construct can be used as an internal standard for expression of mRNA injected into Xenopus oocytes.
Linkage has been established between Waardenburg syndrome type 1 and ALPP in five families (LOD score 4.76 at a recombination fraction of 0.023.
Waldenburg syndrome shows close linkage to FN1 and ALPP in one multigenerational family with 11 affected individuals.
The described RsaI polymorphism was established using a 1.1 kb EcoRI/ApaI fragment of the ALPP cDNA (ALP-1).
Constructed from pBC12/PLAP containing the complete cDNA for the membrane-anchored form by inserting a termination codon after the codon for amino acid 489. The EcoRI/KpnI fragment was then cloned into the vector.
References

Berger J, et al. Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene epxression in eukaryotic cells. Gene 66: 1-10, 1988. PubMed: 3417148

Tate SS, et al. Secreted alkaline phosphatase: an internal standard for expression of injected mRNAs in the Xenopus oocyte. FASEB J. 4: 227-231, 1990. PubMed: 2298343

Penhallow RC, Sussman HH. Further characterization of SacI RFLPs at the acid alpha glucosidase (GAA) locus. Nucleic Acids Res. 18: 1930, 1990. PubMed: 1970872

Foy C, et al. Assignment of the locus for Waardenburg syndrome type 1 to human chromosome 2q37 and possible homology to the splotch mouse. Am. J. Hum. Genet. 46: 1017-1023, 1990. PubMed: 2339698

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479
  • 曹經理
    手機號：18662422060
    QQ：3671869992