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p67-10

規(guī)格:

價(jià)格:詢價(jià)

編號(hào):B183442

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

p67-10

商品貨號(hào)

B183442

Designations

p67-10

GenBank Number

M32011

培養(yǎng)基

Species

Homo sapiens, human

Depositors

TL Leto

Applications

produces protein neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) neutrophil cytosolic factor 2

Vector

Construct size (kb): 5.199999809265137

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: NCF2
Genomic copy number: unique
Gene name: neutrophil cytosolic factor 2 (65kD, chronic granulomatous
disease, autosomal 2)
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 cen-q32
Tissue: HL-60 cell line (promyelocytic), differentiated
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.16
Cross references: DNA Seq. Acc.: M32011

Insert lengths(kb): 2.160000085830689

Tissue: HL-60 cell line (promyelocytic), differentiated

Gene product: neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)(neutrophil cytosolic factor 2) [NCF2]

Alleles: A1, A2

Target Gene: neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)

Insert Size (kb)

2.160

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.1; SstI--3.7, 0.9, 0.41; BamHI--4.0, 1.0; BglII--5.1; PstI--5.1.

The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.84, 1.22; BamHI--0.97; BglII--0.78; SmaI--1.17.

Contains the complete coding sequence (nt 68-1648).

Detects a transcript of 2.4 kb.

The polymorphism was detected with a 0.84 kb EcoRI/SacI fragment.

Enzymes(s) not detecting polymorphism: AvaI, BamHI, EcoRI, KpnI, PstI, SacI, XhoI.

References

Francke U, et al. Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1). Am. J. Hum. Genet. 47: 483-492, 1990. PubMed: 2393022

Leto TL, et al. Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60(c-src). Science 248: 727-730, 1990. PubMed: 1692159

Kenney RT, Leto TL. A HindIII polymorphism in the human NCF2 gene. Nucleic Acids Res. 18: 7193, 1990. PubMed: 1979859

B174672：p67-10

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