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3T3-L1 MBX
3T3-L1 MBX
規(guī)格:
價(jià)格:詢(xún)價(jià)
編號(hào):B183606
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
3T3-L1 MBX
商品貨號(hào)
B183606
Organism
Mus musculus, mouse
Tissue
embryo
培養(yǎng)基
Cell Type
fibroblast
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
adipogenesis and adipocyte differentiation,  diabetes and obesity research areas
Storage Conditions
liquid nitrogen vapor phase
Derivation
3T3 L1-MBX clone was derived from 3T3-L1 (CL-173) to ensure close to 100% differentiation to adipocytes and great response to insulin
Comments

The 3T3 L1-MBX clone was derived from 3T3-L1 (ATCC CL-173) to ensure close to 100% differentiation to adipocytes and great response to insulin. In addition, 3T3 L1-MBX has a great insulin-stimulated glucose uptake response which is about 8-10 fold window with sub-maximal insulin concentration in 2-deoxyglucose uptake assay (2-DOG). This cell line would be a valuable tool for researchers who are interested in diabetes and obesity research areas.

Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Note: Never allow culture to become completely confluent.

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels. 
    The recommended inoculum is 3 to 5 X 103 cells/cm2. Subculture before cultures become 80 to 90% confluent.
Interval: Every three days
Medium Renewal: 2 to 3 times per week

Cryopreservation
90% FBS supplemented with 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor
CymaBay Therapeutics (formerly Metabolex, Inc.) & Choi Y
References

Gregoire FM, et al. MBX-102/JNJ39659100, a novel peroxisome proliferator-activated receptor-ligand with weak transactivation activity retains antidiabetic properties in the absence of weight gain and edema. Mol. Endocrinol. 23(7): 975-88, 2009. PubMed: 19389808

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