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Quantitative Synthetic Eastern equine encephalitis virus RNA

規格:

貨期:

編號:B183890

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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產品名稱

Quantitative Synthetic Eastern equine encephalitis virus RNA

商品貨號

B183890

Agent

Quantitative Synthetic Eastern equine encephalitis virus RNA

Applications

ATCC^® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen
Specification range: 1 x 10⁵ to 1 x 10⁶ copies/µL
100 μL per vial with Biomatrica RNAstable

Storage Conditions

-70°C or colder

Intended Use

The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.

Comments

Manufactured under ISO 13485 guidance

Preparation includes fragments from capsid, envelope (E1 and E2), and nonstructural genes NSP1, NSP3, and 3’UTR.

The following primers and probe can be used with this nucleic acid preparation Ref

Lambert AG, et al. Detection of North American eastern and western equine encephalitis viruses by nucleic acid amplification assays. J. Clin. Microbiol. 41(1): 379-385, 2003. PubMed: 12517876:
Forward primer: ACACCGCACCCTGATTTTACA
Reverse primer: CTTCCAAGTGACCTGGTCGTC
Probe: FAM-TGCACCCGGACCATCCGACCT-BHQ

Special Collection

RNA

References

Lambert AG, et al. Detection of North American eastern and western equine encephalitis viruses by nucleic acid amplification assays. J. Clin. Microbiol. 41(1): 379-385, 2003. PubMed: 12517876

Zink SD, et al. Quadraplex qRT-PCR assay for the simultaneous detection of Eastern equine encephalitis virus and West Nile virus. Diagn. Microbiol. Infect. Dis. 77(2): 123-132, 2013. PubMed: 23891222

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