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CD43-Afl3.6
CD43-Afl3.6
規格:
價格:詢價
編號:B186541
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CD43-Afl3.6
商品貨號
B186541
Designations
CD43-Afl3.6
GenBank Number

J04168

培養基
Species
Homo sapiens, human
Depositors
J Frelinger
Vector
Construct size (kb): 4.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 1.200000047683716
Tissue: peripheral blood cells (PHA activated)
Gene product: sialophorin (gpL115, leukosialin, CD43) [SPN]
Insert Size (kb)
1.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The insert contains BamHI sites at positions 315 and 471 of the published sequence.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.2; AvaI--3.1, 1.2; BamHI--4.1, 0.2; KpnI--4.2; XbaI--4.2.
The insert extends from about position 248 to the AflII site at 1294 of the published sequence. The coding sequence extends form 96 to 1295. This partial cDNA encodes part of the extracellular and all of the transmembrane and intracellular domains.
The insert detects mRNA of 8.0 kb and 2.3 kb in K562, Jurkat, HL-60, and U937 cell lines.
The HincII site of the vector and the AflII site of the insert were destroyed in cloning. The EcoRI site is at the 5' end. Excision with AvaI cuts at the SmaI and XhoI sites of the polylinker.
References

Pallant A, et al. Characterization of cDNAs encoding human leukosialin and localization of the leukosialin gene to chromosome 16. Proc. Natl. Acad. Sci. USA 86: 1328-1332, 1989. PubMed: 2521952

John Frelinger, personal communication

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