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當(dāng)前位置: 首頁 > ATCC代理 > pCT29-8 B187135
pCT29-8
pCT29-8
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B187135
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pCT29-8
商品貨號(hào)
B187135
Designations
pCT29-8
GenBank Number

X12548

培養(yǎng)基
Species
Homo sapiens, human
Depositors
R Pohlmann
Applications
Studies of processing in this system suggest that the enzyme is synthesized and transported to lysosomes as a transmembrane protein with a short cytoplasmic tail.
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 2.111999988555908
Tissue: placenta
Gene product: acid phosphatase 2, lysosomal [ACP2]
Insert Size (kb)
2.112
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
PstI digestion also results in a 75 bp fragment not visible under standard conditions.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.18; BamHI--4.55, 0.58; KpnI--uncut; HindIII--5.2; PstI--3.5, 1.08, 0.64.
Insert encodes a 12 bp 5' untranslated region followed by a 1269 bp coding region.
When the insert was cloned into pSVL and transfected into COS cells, acid phosphatase activity was expressed and 40% was membrane-associated.
The EcoRI insert of pCT29-8 was inserted into pBEH and transfected into BHK cells. Studies of processing in this system suggest that the enzyme is synthesized and transported to lysosomes as a transmembrane protein with a short cytoplasmic tail.
The insert detects a 2.3 kb mRNA in human liver and a 21 kb EcoRI fragment in human DNA. It detects a 5.0 kb EcoRI fragment in mouse DNA.
References

Waheed A, et al. Human lysosomal acid phosphatase is transported as a transmembrane protein to lysosomes in tranfected baby hamster kidney cells. EMBO J. 7: 2351-2358, 1988. PubMed: 3056714

Pohlmann R, et al. Human lysosomal acid phosphatase: cloning, expression and chromosomal assignment. EMBO J. 7: 2343-2350, 1988. PubMed: 3191910

Regina Pohlmann, personal communication

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