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pCMV5FIX
pCMV5FIX
規(guī)格:
價格:詢價
編號:B187667
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pCMV5FIX
商品貨號
B187667
Designations
pCMV5FIX
GenBank Number

J00136

培養(yǎng)基
Species
Homo sapiens, human
Applications
in another host, produces protein coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B)
Vector
Construct size (kb): 7.400000095367432
Insert
DNA: cDNA

Insert information:

Insert size (kb): 2.8

Gene : F9, coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B)

DNA : cDNA

Source : Human

Genbank accession : J00136

Insert ends : BamHI, XbaI

Insert lengths(kb): 2.799999952316284
Gene product: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) [F9]
Alleles: A1, A2, D2, C1, D1, C2
Target Gene: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B)
Insert Size (kb)
2.800
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI/BglII--4.6, 2.9; BamHI--7.6; BglII--7.6; EcoRI--4.9, 2.7.
Digestion with EcoRI produces fragments of 4.9 and 2.5 kb.
Bacteria may grow slowly due to the high plasmid copy number.
Corresponds to the wild-type sequence.
References

Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778

Cheung WF, et al. The binding of human factor IX to endothelial cells is mediated by residues 3-11. J. Biol. Chem. 267: 20529-20531, 1992. PubMed: 1400370

Cheung WF, et al. The role of the epidermal growth factor-1 and hydrophobic stack domains of human factor IX in binding to endothelial cells. J. Biol. Chem. 266: 8797-8800, 1991. PubMed: 1851159

Wing-Fai Cheung, personal communication

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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