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pFa6a-KANMX6-pADH1-GST

規(guī)格:

價(jià)格:

編號:B189614

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pFa6a-KANMX6-pADH1-GST

商品貨號

B189614

Designations

pFa6a-KANMX6-pADH1-GST

Depositors

DJ DeMarini

培養(yǎng)基

Biosafety Level

Vector Information

Vector: pFa6a-KANMX6-pADH1-GST (plasmid)

Construction: pCRTOPO2.1

Marker(s):ampR

Construct size (kb): 5.557000160217285

Applications

cloning vector

Comments

Restriction digests of the clone give the following sizes (kb): BglII--5.6; EcoRI--5.6; BglII/EcoRI--4.4, 1.3.

The marker, promoter and GST cassette is flanked by the F4 primer site (5') and the R4 primer site (3'). The size of the F4/R4 PCR product is 2692 nt.

F4 primer sequence: 5'-(gene-specific sequence)GAATTCGAGCTCGTTTAAAC-3'. R6 primer sequence: 5'-(gene-specific sequence) CATTGTATATGAGATAGTTGA-3'.

R4 primer sequence: 5'-(gene-specific sequence) ACGCGGAACCAGATCCGATT-3'. The gene-specific sequences of these primers are chosen to correspond to the reverse complement of the N-terminal codons of the target gene.

Contains a functional truncated (700 nt) ADH promoter. The promoter is not down regulated during the exponential phase.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

DeMarini DJ, et al. Constitutive promoter modules for PCR-based gene modification in Saccharomyces cerevisiae. Yeast 18: 723-728, 2001. PubMed: 11378899