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HFBEB19

規格:

價格:

編號:B189923

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

HFBEB19

商品貨號

B189923

Designations

HFBEB19

GenBank Number

X63527

unsubmitted

T06940

培養基

Species

Homo sapiens, human

Vector

Construct size (kb): 4.900000095367432

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RPL19
Gene name: ribosomal protein L19
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 p12-q11
Genome: Homo sapiens
Gene name: dihydrolipoamide S-acetyltransferase
Contains complete coding sequence?: U
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.91
Cross references: DNA Seq. Acc.: T06940; EST: EST04829; Primer: M13-21
DNA Seq. Acc.: X63527; EST: EST04829
Nucleotides 1-217 of the insert correspond to
nucleotides 691-475 of X63527.
DNA Seq. Acc.: unsubmitted; EST: EST07647; THC id:
THC121801

Insert lengths(kb): 1.9099999666214

Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)

Gene product: dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)( M2 antigen complex 70 kDa subunit, dihydrolipoamide S-acetyltransferase) [RPL19]

Insert Size (kb)

1.910

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 0.88, 0.66, 0.37.

References

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

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