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pCMV-SREBP1c-436
pCMV-SREBP1c-436
規(guī)格:
價(jià)格:
編號:B190060
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pCMV-SREBP1c-436
商品貨號
B190060
Designations
pCMV-SREBP1c-436
GenBank Number

S66167S1

培養(yǎng)基
Species
Homo sapiens, human
Depositors
JD Horton, H Shimano, JL Goldstein
Applications
contains sequence sterol regulatory element binding transcription factor 1 SREBF1 sterol regulatory element binding transcription factor 1, SREBP1, sterol regulatory element binding protein-1
Vector
Construct size (kb): 6.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCMV7
Intact vector size: 4.900
Type of vector: phagemid
Vector end: SmaI
Vector end: SmaI
Cloning sites: PstI, SalI, XbaI, BamHI, SmaI
Polylinker sites: PstI, SalI, XbaI, BamHI, SmaI
Features (with orientation and position when available):
enhancer: N701
MCS: PstI...SmaI
coding sequence: hGH
enhancer: SV40
marker(s): ampR, <-
replicon: f1, <-
promoter: CMV
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: SREBF1 sterol regulatory element binding transcription
factor 1
Contains complete coding sequence?: N
Chromosome: 17; Localization: 17 p11.2-p11.2
Type of DNA: cDNA
Insert end: SalI-blunted
Insert end: SalI-blunted
Insert size (kb): 1.4
Cross references: DNA Seq. Acc.: S66167S1
Insert lengths(kb): 1.399999976158142
Gene product: sterol regulatory element binding transcription factor 1( SREBF1 sterol regulatory element binding transcription factor 1, SREBP1, sterol regulatory element binding protein-1)
Target Gene: sterol regulatory element binding transcription factor 1
Insert Size (kb)
1.400
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.3; SalI--4.9, 1.4; PstI--5.8, 0.35, 0.33; PstI/XbaI--4.9, 0.9, 0.5; XbaI--6.3.
Insert encodes amino acids 1-436 of human SREBP-1c cDNA and 5' UTR.
Insert sequence also corresponds to Genbank U00968.
References

Shimano H, et al. Isoform 1c of sterol regulatory element binding protein is less active than isoform 1a in livers of transgenic mice and in cultured cells. J. Clin. Invest. 99: 846-854, 1997. PubMed: 9062341

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