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pBYr5aSP

規(guī)格:

價格:詢價

編號:B190987

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pBYr5aSP

商品貨號

B190987

Designations

pBYr5aSP

Species

Bean yellow mosaic virus

培養(yǎng)基

Depositors

J Hammond

Vector

Construct size (kb): 3.75

Insert

DNA: cDNA

Insert lengths(kb): 0.75

Gene product: genome, partial

Insert Size (kb)

0.75

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

The insert has an internal HindIII site about 180 bp from the vector EcoRI site. The insert also includes DdeI sites (2), TaqI sites (2), Sau3A1 istes (2), and a RsaI site.

Restriction digests of the clone give the following sizes (kb): PstI--3.0, 0.75; EcoRI--3.8; HindIII--3.2, 0.6.

Also available from the ATCC are pBYr5 (ATCC 45074) and pBYr5bSP (ATCC 45076) which contain the 0.75 kb PstI fragment. The former is cloned in pUC9 and the latter is also in pSP64, but in the opposite orientation.

pBYr5aSP was constructed by random priming.

RNA transcribed from the SP6 promoter is the virion sense strand, and will hybridize to (-) strand RNA.

Classification

Potyvirus

References

John Hammond, personal communication

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