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當(dāng)前位置: 首頁(yè) > ATCC代理 > 2+6+11 B191372
2+6+11
2+6+11
規(guī)格:
價(jià)格:
編號(hào):B191372
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
2+6+11
商品貨號(hào)
B191372
Designations
2+6+11
GenBank Number

Z22551

培養(yǎng)基
Species
Homo sapiens, human
Depositors
D Adam
Applications
contains sequence kinectin
Vector
Construct size (kb): 7.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-7Zf(+)
Intact vector size: 3.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII
HindIII BamHI SacI BstXI NsiI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: KIN*
Gene name: kinectin
Contains complete coding sequence?: Y
Type of DNA: cDNA
Insert end: Modification: EcoRI adaptor
Insert end: Modification: EcoRI adaptor
Insert size (kb): 4.309
Cross references: DNA Seq. Acc.: Z22551
Nucleotides 1-4309 of the insert correspond to
nucleotides 1-4309 of Z22551.
Insert lengths(kb): 4.309000015258789
Gene product: kinectin [KIN*]
Target Gene: kinectin
Insert Size (kb)
4.309
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.0, 1.0; EcoRI--4.7, 3.4; XhoI--4.4, 3.7.
Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.85; BglII--3.76; HindIII--0.20, 0.70, 1.67; KpnI--0.62; NcoI--0.07; PstI--1.40, 3.21; PvuII--0.54, 2.95; XbaI--3.42; XhoI--0.31.
Recommendation for verification: EcoRI--4.3, 3.0; BamHI--6.4, 0.9; HindIII--5.6, 1.0, 0.5, 0.2; XbaI--6.4, 0.9; XhoI--4.0, 3.3.
References

Futterer A, et al. Molecular cloning and characterization of human kinectin. Mol. Biol. Cell 6: 161-170, 1995. PubMed: 7787243

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