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42TA
42TA
規格:
價格:詢價
編號:B191405
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
42TA
商品貨號
B191405
Organism
Ictalurus punctatus, channel catfish
Tissue
peripheral blood
培養基
Cell Type
macrophage
Product Format
frozen
Morphology
macrophage
Culture Properties
mixed, adherent and suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
normal
Storage Conditions
liquid nitrogen vapor phase
Karyotype
diploid
Images
Derivation
The 42TA cell line was developed in 1992.The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors.
Comments
The 42TA cell line was developed in 1992.The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors.
Complete Growth Medium
A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 10% double distilled water and 5% heat-inactivated catfish serum.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml. Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.
Attached cells may be subcultured by scraping.
Cryopreservation
Freeze medium: Fetal bovine serum supplemented with 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 27.0°C
Name of Depositor
NW Miller
Deposited As
Ictalurus punctatus
Year of Origin
1992
References

Miller N, et al. Functional and molecular characterization of teleost leukocytes. Immunol. Rev. 166: 187-197, 1998. PubMed: 9914913

Miller NW, et al. Development of leukocyte cell lines from the channel catfish (Ictalurus punctatus). J. Tissue Culture Methods 16: 117-123, 1994.

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