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lambdaYES-R
lambdaYES-R
規(guī)格:
價(jià)格:
編號(hào):B191756
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
lambdaYES-R
商品貨號(hào)
B191756
Designations
lambdaYES-R
Depositors
SJ Elledge
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 42.6
DESCRIPTION OF VECTOR:
Intact vector size: 42.600
Type of vector: phage
Cloning sites: XhoI, EcoRI
Polylinker sites: EcoRI XhoI EcoRI
Construction: lambdagt6; pSE936
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: GAL1, ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: lox, ->
other: right arm (15.2 kb, with cI857)
Vector: lambdaYES-R (phage, lambda - replacement)
Promoters: Promoter for expression GAL1
Construction: lambdagt6; pSE936
Marker(s):URA3,ampR
Construct size (kb): 42.6
Features: marker(s): URA3
marker(s): ampR
other: left arm (19.6 kb)
other: lox
other: right arm (15.2 kb, with cI857)
promoter for expression: GAL1
promoter for expression: lac
replicon: ARS1
replicon: pMB1
MCS: XhoI EcoRI
transcription terminator: HIS3
centromere: CEN4
Applications
expression vector
lambda vector allowing conversion to plasmid
vector for constructing cDNA libraries
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--24.0, 20.0; NotI--44.0; XhoI--24.0, 20.0.
The plasmid derivative (pSE936, 7.8 kb) is a YC-type shuttle expression vector.
Transcription initiated from the GAL1 promoter in yeast hosts can be terminated at the HIS3 terminators. GAL1 does not provide translational start signals.
Vector for the construction of cDNA libraries permitting regulated expression in both yeast and E. coli and simple conversion to plasmid via the cre-lox system.
The lac promoter contains a ribosome binding sequence optimally spaced from an AUG start codon and can support translational fusions.
Growth Conditions
Temperature: 37.0°C
References

Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010

Stephen J Elledge, personal communication

Shipped
frozen
Shipping Information
Distributed: frozen bacteria-free lysate
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