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CRI-C44
CRI-C44
規(guī)格:
價(jià)格:
編號(hào):B193030
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CRI-C44
商品貨號(hào)
B193030
Designations
CRI-C44
Species
Homo sapiens, human
培養(yǎng)基
Depositors
Collaborative Research, Inc.
Vector
Construct size (kb): 36.5
Insert
DNA: genomic
Insert lengths(kb): 30.0
Gene product: DNA Segment, single copy [D5S56]
Insert Size (kb)
30.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--13.0, 7.4, 6.5, 5.0, 4.2, 3.7, 0.7; HindIII--7.4, 7.4, 6.5, 5.9, 3.6, 2.6, 2.1, 1.95, 0.7; BglII--11.0, 6.5, 5.8, 4.3, 4.1, 2.7, 0.7;
PstI--6.5, 5.0, 3.6, 2.8, 2.3, 2.1, 2.0, 1.6, 1.5, 1.4, 1.2, 1.1, 1.0, 0.8, 0.6, 0.5.
Insert fragments can be separated from the vector with an EcoRI digest. The vector fragment is 5.5 kb.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Enzyme(s) not detecting polymorphism: PstI (panel of 5 individuals).
References

Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638

Bowden DW, et al. Identification and characterization of 23 RFLP loci by screening random cosmid genomic clones. Am. J. Hum. Genet. 44: 671-678, 1989. PubMed: 2565079

Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192

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