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pBS-human p57 [KIP2]

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編號:B195133

品牌:Mingzhoubio

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RNA

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產品名稱

pBS-human p57 [KIP2]

商品貨號

B195133

Designations

pBS-human p57 [KIP2]

GenBank Number

U22398

培養基

Species

Homo sapiens, human

Depositors

SJ Elledge

Vector

Construct size (kb): 4.460000038146973

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDKN1C
Other gene symbols: KIP2
Gene name: cyclin-dependent kinase inhibitor 1C (p57, Kip2)
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11 p15.5
Tissue: embryo
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: U22398
Nucleotides 1-1511 of the insert correspond to
nucleotides 1-1511 of U22398.

Insert lengths(kb): 1.5

Tissue: embryo

Gene product: cyclin-dependent kinase inhibitor 1C (p57, Kip2)(P57, kinase inhibitory protein 2 of cyclins (p57)) [KIP2]

Insert Size (kb)

1.5

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--4.6; EcoRI--3.0, 1.6; EcoRI/HindIII--3.0, 1.55; EcoRI/PstI--3.0, 1.15, 0.47; PstI--3.5, 1.15; SmaI--3.2, 1.4.

The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.4, 0.49; HindIII--1.44; SmaI--0.25.

References

Matsuoka S, et al. p57KIP2, a structurally distinct member of the p21CIP1 Cdk-inhibitor family, is a candidate tumor suppressor gene. Genes Dev. 9: 650-662, 1995. PubMed: 7729684

B227994：pBS-human p57 [KIP2]

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