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hFGFR-1
hFGFR-1
規(guī)格:
價(jià)格:
編號(hào):B195607
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
hFGFR-1
商品貨號(hào)
B195607
Designations
hFGFR-1
GenBank Number

M34641

培養(yǎng)基
Species
Homo sapiens, human
Applications
in another host, produces protein fibroblast growth factor receptor 1 fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2)
Vector
Construct size (kb): 5.900000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSV7d
Intact vector size: 2.400
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1, SV40 ori
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: FGFR1
Other gene symbols: FLT2
Genomic copy number: unique
Gene name: fibroblast growth factor receptor 1 (fms-related tyrosine
kinase 2)
Contains complete coding sequence?: Y
Chromosome: 8; Localization: 8 p12
Tissue: teratocarcinoma cell line Tera-2 clone 13
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 3.343
Cross references: DNA Seq. Acc.: M34641
Insert lengths(kb): 3.342999935150147
Tissue: teratocarcinoma cell line Tera-2 clone 13
Gene product: fibroblast growth factor receptor 1(fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2)) [FLT2]
Target Gene: fibroblast growth factor receptor 1
Insert Size (kb)
3.343
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 0.65; KpnI--4.0, 1.85; NcoI/XbaI--3.4, 2.35; PstI--2.4, 1.85, 1.6; SstI--5.9.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.32, 1.34, 1.73, 2.76; BglII--0.93, 2.08, 2.69; EcoRI--0.59; KpnI--1.21, 3.01; PstI--0.16, 1.72; PvuII--0.22, 0.73, 2.25, 2.51; SacI--2.35.
Contains the complete coding sequence (nt 10-2472).
A 450 bp fragment from the 5' end (encoding the extracellular domain) detects transcripts of 4.0 kb and 4.8 kb.
The 3' EcoRI site was destroyed during cloning.
References

Wennstrom S, et al. cDNA cloning and expression of a human FGF receptor which binds acidic and basic FGF. Growth Factors 4: 197-208, 1991. PubMed: 1722683

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