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pMR103

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編號:B196275

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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產品名稱

pMR103

商品貨號

B196275

Designations

pMR103

Depositors

L Regan, M Munson

培養基

Biosafety Level

Host

Distribution host: Escherichia coli JM101

Vector Information

Size (kb): 5.0999999046325680

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pMR102
Intact vector size: 4.100
Type of vector: phagemid
Vector end: SalI
Vector end: PstI
Cloning sites: NcoI BamHI
Polylinker sites:
Other unique sites: EcoNI, SalI, BstEII, PstI
Construction: pMR101
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: M13, ->, 11-467
replicon: p15A, ->, 1585-1588
marker(s): kanR, ->, 2179-2994
operator: lac, <-, 3784-3800
promoter for expression: T7 (phi10), <-, 3804-3823
Cross references:

Vector: pMR103 (phagemid)

Promoters: Promoter for expression T7 (phi10)

Construction: pMR102, pET11d

Marker(s):kanR

Construct size (kb): 5.099999904632568

Features: marker(s): kanR
operator: lac
promoter for expression: T7 (phi10)
replicon: M13
replicon: p15A
repressor gene: lacI

Applications

expression vector

vector permitting production of single-stranded DNA

Comments

Restriction digests of the clone give the following sizes (kb): PstI/SalI--5.2; PstI--5.2; SalI--uncut; EcoRV--3.2, 2.0; BstEII--3.6, 1.6; BstEII/PstI--3.0, 1.7, 0.5.

The SalI site was lost during cloning.

The lacI gene permits tight regulation of transcription of an insert and synthesis of potentially toxic proteins.

Expression vector (T7-based) with a kanR marker and a P15A replicon compatible with ColE1-derived plasmids. Particularly useful for co-transformation with ColE1-based ampR T7 expression vectors and the production of two proteins in the same cell.

If used in an Escherichia coli strain that expresses T7 polymerase under the control of the lacUV5 promotor (such as BL21(DE3)), addition of IPTG can result in high levels of recombinant protein production.

Media

ATCC^® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin

Growth Conditions

Temperature: 37.0°C

References

Munson M, et al. ColE1-compatible vectors for high-level expression of cloned DNAs from the T7 promoter. Gene 144: 59-62, 1994. PubMed: 8026759

Shipped

frozen

Shipping Information

Distributed: freeze-dried

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