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c08H4
c08H4
規格:
價格:詢價
編號:B196336
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
c08H4
商品貨號
B196336
Designations
c08H4
Species
Homo sapiens, human
培養基
Depositors
PI Patel
Applications
The 7.5 kb EcoRI fragment of the insert has been used to detect the TaqI RFLP at D17S122 and the dosage difference indicative of the duplication mutation associated with Charcot-Marie-Tooth disease (CMT1A).
Vector
Construct size (kb): 36.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: SuperCos 1
Intact vector size: 7.600
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, neoR, G418R
promoter: T3, T7
replicon: pMB1, SV40
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D17S122
Genomic copy number: unique
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 p12-p11.2
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 29.0
Cross references:
Insert lengths(kb): 29.0
Gene product: DNA Segment, single copy [D17S122]
Alleles: A1, A1, A2, A3, B1, A2, B2
Insert Size (kb)
29.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.4, 7.6, 7.5, 4.4, 2.6, 1.55, 1.45 (doublet); NotI--9.4 (doublet), 7.6, 1.45; BamHI--11.5, 8.0, 6.6, 5.2, 3.5;
EcoRI/BamHI--7.6, 7.5, 5.4, 3.0, 2.7, 2.15, 1.6, 1.45 (doublet); SacII--uncut.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
The 7.5 kb EcoRI fragment of the insert has been used to detect the TaqI RFLP at D17S122 and the dosage difference indicative of the duplication mutation associated with Charcot-Marie-Tooth disease (CMT1A).
References

Lupski JR, et al. DNA duplication associated with Charcot-Marie-Tooth disease type 1A. Cell 66: 219-232, 1991. PubMed: 1677316

Pragna I Patel, personal communication

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