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pEM301 [pHUMDUT1]
pEM301 [pHUMDUT1]
規(guī)格:
價格:
編號:B196559
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pEM301 [pHUMDUT1]
商品貨號
B196559
Designations
pEM301 [pHUMDUT1]
GenBank Number

M89913

培養(yǎng)基
Species
Homo sapiens, human
Depositors
EM McIntosh
Applications
in another host, produces protein dUTP pyrophosphatase
Vector
Construct size (kb): 3.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTZ19R
Intact vector size: 2.900
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC19, Escherichia coli lacZalpha, pBR322, f1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DUT*
Genomic copy number: multiple
Gene name: dUTP pyrophosphatase (dUTPase)
Contains complete coding sequence?: Y
Chromosome: 15; Localization: 15, cR
Type of DNA: cDNA
Insert end: Modification: EcoRI adapters
Insert end: Modification: EcoRI adapters
Insert size (kb): 0.6
Cross references: DNA Seq. Acc.: M89913
Insert lengths(kb): 0.6000000238418579
Gene product: dUTP pyrophosphatase [DUT*]
Target Gene: dUTP pyrophosphatase
Insert Size (kb)
0.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.5; XmnI--2.4, 1.1; SmaI--3.3, 0.15; EcoRI--2.9, 0.6; PstI--3.5.
The insert contains the following restriction sites (nt from the 5' end): SphI--17, 86; SmaI--134.
Contains the complete coding sequence, and corresponds to nt 1-545 of the database record.
The sense strand is packaged.
pEM301 (ATCC 79032/3) and pEM302 (79034/5) differ only in the orientation of the insert.
References

McIntosh EM, et al. Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. Proc. Natl. Acad. Sci. USA 89: 8020-8024, 1992. PubMed: 1325640

Evan M McIntosh, personal communication

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