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pHUCI
pHUCI
規(guī)格:
價格:
編號:B196798
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pHUCI
商品貨號
B196798
Designations
pHUCI
GenBank Number

X07884

培養(yǎng)基
Species
Homo sapiens, human
Depositors
H Kuivaniemi
Vector
Construct size (kb): 7.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC
Intact vector size: 2.700
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: COL1A1
Genomic copy number: unique
Gene name: collagen, type I, alpha 1
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q21.3-q22
Tissue: fibroblast cell line SV80
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 5.2
Cross references: DNA Seq. Acc.: X07884
Nucleotides 1-1500 of the insert correspond to
nucleotides 1-1500 of X07884.
Insert lengths(kb): 5.199999809265137
Tissue: fibroblast cell line SV80
Gene product: collagen, type I, alpha 1 [COL1A1]
Insert Size (kb)
5.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.7, 2.3; EcoRI--4.6, 2.7, 0.64; PvuII--2.5, 2.0, 1.5, 0.65, 0.5, 0.35; SacI--7.0, 0.52; XhoI--4.9, 2.4, 0.7.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--2.9; EcoRI--4.5; KpnI--0.2; NcoI--0.6, 0.7; PvuII--0.6, 0.85, 2.3, 4.3; SacI--1.0, 1.5; XhoI--0.8, 1.5.
To obtain a high yield, grow the culture in minimal media with casamino acids (M9), followed by chloramphenicol amplification to increase the plasmid copy number.
References

Tromp G, et al. Structure of a full-length cDNA clone for the prepro alpha1(I) chain of human type I procollagen. Biochem. J. 253: 919-922, 1988. PubMed: 3178743

Helena Kuivaniemi, personal communication

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