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UPCI:SCC090
UPCI:SCC090
規格:
價格:
編號:B197293
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
UPCI:SCC090
商品貨號
B197293
Organism
Homo sapiens, human
Tissue
Derived from metastatic site: tongue
培養基
Cell Type
squamous cell
Product Format
frozen 1.0 mL
Morphology
epithelial-like
Culture Properties
adherent
Biosafety Level
2  [Cells contain human papillomavirus (HPV) DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
squamous cell carcinoma; grade 3; pathological stage: T2N0
Age
46
Gender
male
Ethnicity
Caucasian
Applications
This cell line represents an important resource for investigation into the tumor biology and the study of genetic abnormalities associated with the development and/or progression of HNSCC.
Storage Conditions
liquid nitrogen vapor phase
Images
Cell Micrograph of ATCC CRL-3239
Derivation
The surgically excised tumor was minced and distributed into flasks. When growth of fibroblasts obstructed the growth of the squamous cells, the fibroblasts were removed by trypsinization. The remaining squamous cells were grown and passaged.
Clinical Data
46 years
Caucasian
male
The donor smoked tobacco, drank alcoholic beverages, and died of his disease
Antigen Expression
CK 19+; Keratin IHC AE1+; Keratin IHC AE3+; CAM 5.2+
Oncogene
amplification of chromosomal band 11q13, not expressed mutations of TP53 codon, not expressed
Comments
UPCI:SCC090 is from a squamous cell carcinoma of the base of the tongue. These cells are positive for Human Papilloma Virus (HPV). They have no TP53 mutations as assayed by sequencing the 5-8 exons of TP53. UPCI:SCC090 showed no amplification of chromosomal band 11q13 using FISH.

The patient had a recurrence about 1 year later sited at the hypopharynx. It is ATCC® No. CRL-3240 UPCI:SCC152™.

These 2 HNSCC (head and neck squamous cell carcinoma) cell lines are a resource for studying the initiation, cancerization, prognosis, intervention and treatment of oral cancers.


Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium:
  • additional 2mM L-Glutamine
    • fetal bovine serum (FBS) to a final concentration of 10%

Seeding Density
4 x 104 viable cells/cm2
Subculturing
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
    1. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC® No. 30-2200) or 0.25% Trypsin – 0.53mM EDTA (ATCC® No. 30-2101) solution to remove all traces of serum which contains trypsin inhibitor.
    2. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    4. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
    Subcultivation Ratio: 1:2 to 1:4 is recommended.
    Medium Renewal: 2 to 3 times a week
Volume
1.0 mL
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D5S818: 11, 12
D7S820: 9, 10
D13S317: 11
D16S539: 12,13
TH01: 7
TPOX:  8
vWA: 17
Name of Depositor
Susanne M. Gollin, University of Pittsburgh
Year of Origin
September 1994
References

White JS, et al. The influence of clinical and demographic risk factors in the establishment of head and neck squamous cell carcinoma cell lines. Oral Oncol. 43:701-712, 2007. PubMed: 17112776

Martin CL, et al. Chromosomal imbalances in oral squamous cell carcinoma: examination of 31 cell lines and review of the literature. Oral Oncol. 44:369-382, 2008. PubMed: 17681875

Ragin C, et al. Mapping and analysis of HPV16 integration sites in a head and neck cancer cell line. Int. J. Cancer 110:701-709, 2004. PubMed: 15146560

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