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pHGH107
pHGH107
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B197716
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pHGH107
商品貨號(hào)
B197716
Designations
pHGH107
Species
Homo sapiens, human
培養(yǎng)基
Depositors
Genentech, Inc.
Applications
produces protein growth hormone 1 growth hormone GH1
Vector
Construct size (kb): 5.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGH6
Intact vector size: 4.600
Type of vector: plasmid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: EcoRI
Polylinker sites:
Construction: pBR322, pKB268
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
promoter: lacUV5
replicon: pMB1
Cross references:
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGH6
Intact vector size: 4.600
Type of vector: plasmid
Vector end: EcoRI
Vector end: HindIII
Cloning sites: EcoRI
Polylinker sites:
Construction: pBR322, pKB268
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
promoter: lacUV5
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: GH1
Genomic copy number: unique
Gene name: growth hormone 1
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 q22-q24
Type of DNA: cDNA
Insert end: EcoRI
Insert end: SmaI
Insert size (kb): 0.591
Cross references:
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: GH1
Genomic copy number: unique
Gene name: growth hormone 1
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 q22-q24
Type of DNA: cDNA
Insert end: EcoRI
Insert end: SmaI
Insert size (kb): 0.591
Cross references:
Insert lengths(kb): 0.5910000205039978
Gene product: growth hormone 1(growth hormone GH1) [GH1]
Target Gene: growth hormone 1
Insert Size (kb)
0.591
Media
ATCC® Medium 1083: LB medium for chi 1776
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.6.
For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L).
The insert contains sequences encoding all 191 amino acids of human growth hormone.
Contains an internal PstI, BglII and PvuII site.
The vector (ATCC 31539, 40012) is pBR322 modified by the insertion of 2 lac UV5 promoters at the EcoRI site. These promoters regulate expression of the insert.
References

Goeddel DV, Heyneker HL. Method of constructing a replicable cloning vehicle having quasi-synthetic genes. US Patent 4,342,832 dated Aug 3 1982

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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