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phpep14-21

規格:

價格:

編號:B199237

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

phpep14-21

商品貨號

B199237

Designations

phpep14-21

Species

Homo sapiens, human

培養基

Vector

Construct size (kb): 5.199999809265137

Insert

DNA: cDNA

Insert lengths(kb): 0.7049999833106995

Tissue: gastric mucosa

Gene product: pepsinogen [PGA*]

Insert Size (kb)

0.705

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.75; HindIII--5.2; EcoRI--5.2; AvaI--5.2; BamHI--5.2.

Detects genomic restriction fragments of the following sizes (kb): owl monkey BamHI--13.0, 3.6.

Insert corresponds to bases 332 - 1017 of predicted 1374 mRNA sequence and amino acids 80 - 307. Includes a portion of exon 3 through exon 8. Exon 3-5 and 6-8 specific probes can be generated by HaeII digestion.

The 17.2 kb EcoRI genomic fragment is detected by the exon 3 - 5 probe but not the 6 - 8 probe. The 17.2 kb EcoRI fragment is detected in haplotypes A (PGA3, PGA4, PGA5 genes) and B (PGA3, PGA4 genes).

The 3.9 kb EcoRI genomic fragment is detected by the exon 6 - 8 probe but not the exon 3 - 5 probe. This fragment is a doublet resulting from two distinct PGA genes.

The 13.5 kb EcoRI genomic fragment is detected by the exon 3 - 5 probe but not the exon 6 - 8 probe. This EcoRI fragment is detected in haplotype A which contains the PGA3, PGA4, and PGA5 genes.

The 22.0 kb and the 17.8 kb genomic EcoRI fragments are detected by both the exon 3 - 5 and 6 - 8 probes, suggesting the size heterogeneity results from variation at the 5' region of two distinct genes.

Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.

References

Ma NS, et al. Homologs of eleven loci on human chromosome 11 assigned to two owl monkey chromosomes. Cytogenet. Cell Genet. 56: 206-211, 1991. PubMed: 2055119

Taggart RT, et al. Variable numbers of pepsinogen genes are located in the centromeric region of human chromosome 11 and determine the high-frequency electrophoretic polymorphism. Proc. Natl. Acad. Sci. USA 82: 6240-6244, 1985. PubMed: 3862130

Taggart RT, et al. Relationships between the human pepsinogen DNA and protein polymorphisms. Am. J. Hum. Genet. 38: 848-854, 1986. PubMed: 3014868

Barbara Weiffenbach, personal communication

B229684：phpep14-21

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