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pHalphaSp5
pHalphaSp5
規格:
價格:詢價
編號:B205025
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pHalphaSp5
商品貨號
B205025
Designations
pHalphaSp5
GenBank Number

M11049

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 5.5
Insert
DNA: cDNA
Insert lengths(kb): 0.699999988079071
Tissue: chronic myelogenous leukemia K-562 cell line
Gene product: spectrin, alpha, erythrocytic 1 (elliptocytosis 2) [SPTA1]
Insert Size (kb)
0.700
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,
Comments
One of the PstI sites flanking the insert was lost in cloning. A PstI/AvaI double digest will excise all of the insert but about 75 bp at the 3' end.
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 0.9; PstI--5.5; PvuII--3.6, 1.9; EcoRI--5.5.
The insert encodes the repeat units alpha14 to alpha18 of the gene.
References

Curtis PJ, et al. Sequence comparison of human and murine erythrocyte alpha-spectrin cDNA. Gene 36: 357-362, 1985. PubMed: 3000887

Huebner K, et al. The alpha-spectrin gene is on chromosome 1 in mouse and man. Proc. Natl. Acad. Sci. USA 82: 3790-3793, 1985. PubMed: 2987946

Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294

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