最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pDS [pDS5] B205342
pDS [pDS5]
pDS [pDS5]
規(guī)格:
價格:
編號:B205342
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pDS [pDS5]
商品貨號
B205342
Designations
pDS [pDS5]
Depositors
H Iba
培養(yǎng)基
Biosafety Level
1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.8499999046325680
DESCRIPTION OF VECTOR:
Intact vector size: 5.850
Type of vector: plasmid
Cloning sites: EcoRV
Polylinker sites:
Cloning capacity: 1.8 kb
Construction: pN4 (src deleted)
Host range: avian cells; Escherichia coli
Features (with orientation and position when available):
restriction site: SalI
coding sequence: 3' env
other: splice acceptor site
restriction site: EcoRV
other: 3' LTR
restriction site: SalI
marker(s): ampR, ->
replicon: pMB1
Vector: pDS (plasmid)
Construction: pN4 (src deleted)
Marker(s):ampR
Construct size (kb): 5.849999904632568
Features: marker(s): ampR
other: 3' LTR
other: splice acceptor site
replicon: pMB1
restriction site: EcoRV
restriction site: SalI
coding sequence: 3' env
Applications
retroviral expression vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRV - 5.9; SalI - 3.3, 2.7; HindIII - 5.9.
Avian retroviral expression vector derived from Rous sarcoma virus (RSV), requiring vector pRep(A) (ATCC 87702) for recombinant virus production.
Replication- competent virus vectors expressing foreign DNA cloned into the EcoRV site can be recovered from the culture fluids after SalI digestion, ligation to SalI digested pRep (A)DNA and transfection into chicken primary cells or appropriate cells
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Murakami M, et al. High-level expression of exogenous genes by replication-competent retrovirus vectors with an internal ribosomal entry site. Gene 202: 23-29, 1997. PubMed: 9427541

Iba H, et al. Transforming potential and growth stimulating activity of the v-fos and c-fos genes carried by avian retrovirus vectors. Oncogene Res. 2: 121-133, 1988. PubMed: 2851123

Yokouchi Y, et al. Misexpression of Hoxa-13 induces cartilage homeotic transformation and changes cell adhesiveness in chick limb buds. Genes Dev. 9: 2509-2522, 1995. PubMed: 7590231

Related Products
component of:ATCC 87703
Shipped
freeze-dried
Shipping Information
Distributed: freeze-dried
  • 王經(jīng)理
    手機號：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機號：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機號：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機號：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機號：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機號：13626845108
    QQ：972239479