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pTSV-NS5

規格:

價格:

編號:B206362

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pTSV-NS5

商品貨號

B206362

Designations

pTSV-NS5

Species

Tobacco streak virus

培養基

Depositors

TJ Morris

Applications

This clone is useful in hybridization tests for the detection of tobacco streak virus (necrotic shock) infection in Fragaria spp.

Vector

Construct size (kb): 3.0

Insert

DNA: cDNA

Insert lengths(kb): 0.3199999928474426

Gene product: genome, partial

Insert Size (kb)

0.320

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): PstI--2.8, 0.34; BamHI--3.2; EcoRI--3.2.

This clone is useful in hybridization tests for the detection of tobacco streak virus (necrotic shock) infection in Fragaria spp.

Insert hybridizes with NS (necrotic shock) RNA 3 from strawberry and R RNA 3 from blackberry but not to R RNA 4 in northern blots of genomic RNA.

Isolated from Fragaria vesca. cDNA was synthesized from virion genomic RNA, dC-tailed and cloned into the dG-tailed PstI site of pUC8.

Classification

Ilarvirus

References

Stenger DC, et al. Characterization and detection of the strawberry necrotic shock isolate of tobacco streak virus. Phytopathology 77: 1330-1337, 1987.

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