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p184
p184
規格:
價格:
編號:B207315
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
p184
商品貨號
B207315
Designations
p184
GenBank Number

S53291

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 3.86899995803833
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-7Zf
Intact vector size: 3.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: XbaI
Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI
HindIII BamHI SacI BstXI NsiI
Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI
HindIII BamHI SacI BstXI NsiI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ADRB3
Gene name: adrenergic, beta-3-, receptor
Contains complete coding sequence?: U
Localization: 8 p12-p11.1
Tissue: SK-N-MC cells
Type of DNA: cDNA
Insert 5' end: EcoRI
Insert 3' end: XbaI
Insert size (kb): 0.869
Cross references: DNA Seq. Acc.: S53291
Insert lengths(kb): 0.8690000176429749
Tissue: SK-N-MC cells
Gene product: adrenergic, beta-3-, receptor [ADRB3]
Insert Size (kb)
0.869
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 0.5, 0.4; BglI--1.54, 1.32, 0.9, 0.04; EcoRI--3.87; EcoRI/XbaI--3.0, 0.87; NcoI--3.44, 0.43.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.36; BglI--0.07, 0.11; NcoI--0.29, 0.72; SacI--0.55, 0.78.
Corresponds to nt 1137-2006 of GenBank accession X70811, spanning a region that contains an intron in the genomic sequence.
Constructed after amplification from cDNA with these primer/adapters: 5'-TGCGAATTCTGCCTTCAACCCGCTC-3'; 5'-ACTATAGGGTCTAGAGGATCCG-3'. The product was digested with EcoRI + XbaI.
Transcription from the SP6 promoter gives the sense strand.
References

Granneman JG, et al. Rodent and human beta 3-adrenergic receptor genes contain an intron within the protein-coding block. Mol. Pharmacol. 42: 964-970, 1992. PubMed: 1336117

James G Granneman, personal communication

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