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pSHT

規格:

價格:

編號:B207991

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pSHT

商品貨號

B207991

Designations

pSHT

Depositors

P Bird

培養基

Biosafety Level

Vector Information

Size (kb): 4.0000000000000000

Vector: pSHT (phagemid)

Promoters: Promoter T7

Construction: pSVT7

Marker(s):ampR

Construct size (kb): 4.0

Features: marker(s): ampR
promoter: SV40 early, T7
replicon: pMB1, M13, SV40

Applications

expression vector

vector permitting production of single-stranded DNA

vector with sequences for protein export

Comments

Restriction digests of the clone give the following sizes (kb): SmaI--4.0; BamHI--4.0; HindIII--4.0.

Shuttle expression vector providing a start codon and signal sequence (from influenza virus hemagglutinin HA) necessary to express membrane and secretory proteins in mammalian cells.

Contains stop codons in all three frames after cloning sites.

The order of the major features in the phagemid is: pMB1 ori - ampR -

M13 ori - ClaI - SV40 ori - SV40 early promoter - T7 promoter - HindIII - HA signal sequence - BamHI/SmaI/SpeI - termination signals - EcoRI - NheI - SV40 intron - BclI - SV40 polyadenylation.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Madison EL, Bird P. A vector, pSHT, for the expression and secretion of protein domains in mammalian cells. Gene 121: 179-180, 1992. PubMed: 1427094

Bird P, et al. Translocation in yeast and mammalian cells: not all signal sequences are functionally equivalent. J. Cell Biol. 105: 2905-2914, 1987. PubMed: 3320059

Shipped

freeze-dried

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