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pLIN101

規格:

價格:

編號:B208708

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pLIN101

商品貨號

B208708

Designations

pLIN101

GenBank Number

U15050

培養基

Species

Streptococcus agalactiae Lehmann and Neumann

Depositors

DG Pritchard, B Lin

Applications

produces protein hyaluronate lysase

Vector

Construct size (kb): 7.931000232696533

Insert

DNA: genomic

Insert lengths(kb): 3.420000076293945

Gene product: hyaluronate lysase [hylB]

Target Gene: hyaluronate lysase

Insert Size (kb)

3.420

Media

ATCC^® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): SalI--8.0; XbaI--8.0; EcoRI--8.0; BamHI--8.0; SalI/XbaI--4.6, 3.4.

The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.35, 0.88; KpnI--1.2; EcoRI--2.19; ClaI--3.22.

The insert excised with SalI/XbaI will include some vector sequence: 12 bp at the 5' end (SalI) and 36 bp at the 3' end (XbaI).

References

Lin B, et al. Cloning and expression of the gene for group B streptococcal hyaluronate lyase. J. Biol. Chem. 269: 30113-30116, 1994. PubMed: 7982914

Pritchard DG, et al. Characterization of the group B streptococcal hyaluronate lyase. Arch. Biochem. Biophys. 315: 431-437, 1994. PubMed: 7986088

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