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pBamRM5.0 [NEB699]

規格:

價格:

編號:B211305

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pBamRM5.0 [NEB699]

商品貨號

B211305

Designations

pBamRM5.0 [NEB699]

GenBank Number

X55285

培養基

Species

Bacillus amyloliquefaciens (Fukumoto) Priest et al.

Depositors

New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.

Applications

produces protein modification methylase BamHI

produces protein restriction endonuclease BamHI

Vector

Construct size (kb): 4.5

Insert

DNA: genomic

Insert lengths(kb): 1.799999952316284

Gene product: modification methylase BamHI [bamHIC]

Target Gene: modification methylase BamHI, restriction endonuclease BamHI

Insert Size (kb)

1.800

Media

ATCC^® Medium 1949: LB medium (ATCC medium 1065) with 50 mcg/ml chloramphenicol and 100 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of pBamRM5.0 give the following sizes (kb): HindIII/SmaI--5.0, 4.4; HindIII--5.0, 4.4; PvuII--4.5, 4.5, 0.4; EcoRI--9.5.

Restriction digests of pBamM1.8 give the following sizes (kb)(faint): HindIII/SmaI--4.4; HindIII--4.4; PvuII--2.4, 2.1; EcoRI--4.5.

References

Brooks JE. Method for producing the BamHI restriction endonuclease and methylase. US Patent 5,137,823 dated Aug 11 1992

Brooks JE, et al. Cloning the BamHI restriction modification system. Nucleic Acids Res. 17: 979-997, 1989. PubMed: 2537955

Brooks JE, et al. Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts. Nucleic Acids Res. 19: 841-850, 1991. PubMed: 1901989

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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