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pSE1112

規格:

價格:詢價

編號:B211691

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pSE1112

商品貨號

B211691

Designations

pSE1112

Species

Homo sapiens, human

培養基

Depositors

SJ Elledge

Applications

in another host, produces protein SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase

Vector

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pAS1
Intact vector size: 7.100
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: NdeI NcoI SfiI SmaI BamHI SalI
Polylinker sites: NdeI NcoI SfiI SmaI BamHI SalI
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: ADC1 (ADH), ->
other: GAL4 DNA-binding domain, ->
epitope tag: hemagglutinin (HA), ->
restriction site: EcoRI, ->, 4315
MCS: NdeI...SalI, ->, 4395-4422
terminator: ADC1
replicon: pMB1
marker(s): ampR
replicon: 2 micron
marker(s): TRP1, ->
replicon: f1
Cross references:

Insert

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: SNF1
Gene name: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 2.3
Cross references:

Insert lengths(kb): 2.299999952316284

Gene product: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase [SNF1]

Target Gene: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase

Insert Size (kb)

2.300

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--9.4; HindIII--5.8, 3.4; BamHI--7.0, 2.3.

pSE1111 (ATCC 87010) and pSE1112 (ATCC 87011) can be used together as a positive control in 2-hybrid cloning systems.

Expresses SNF1 fused to the GAL4 DNA-binding domain.

References

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

Stephen J Elledge, personal communication

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